𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genital human papilloma virus infection in oslo studied by dot blot DNA hybridization and the polymerase chain reaction

✍ Scribed by Kirsti Gjøen; Jens C. Siebke; Magne Flikke; Renate Háger; Gudvor Ertzeid; Arne Halsos; Josef Ekgren; Berit Norling; Bjørn Grinde; Ivar Ørstavik

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
831 KB
Volume
34
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Samples from patients with genital condyloma acuminata or with cervical condylomas and/or dysplasia and from women without cytological/ clinical evidence of cervical affection were examined by dot blot DNA hybridization or the polymerase chain reaction (PCR). The PCR was much more sensitive than dot blot, more than doubling the human papilloma virus (HPV) findings. HPV DNA, mainly HPV 6/11, was detected in 18 of 19 biopsies of condyloma acuminata, whereas HPV 16 was most frequently detected in the 21 cervices (76%) with condyloma and/or dysplasia. HPV 16 was detected in eight of 103 cervical smears with no signs of infection. The prevalence of HPV 16 in cervical samples was somewhat higher than expected. This suggests that, in Oslo, HPV 16 is a common HPV type in women with cytologically normal cervices. HPV 18 was relatively rare and was detected only in combination with other HPVs.

📜 SIMILAR VOLUMES

Large-scale screening for human parvovir
Large-scale screening for human parvovirus B19 DNA in clinical specimens by dot blot hybridization and polymerase chain reaction
✍ Yuko Yoto; Tooru Kudoh; Keiji Haseyama; Nobuhiro Suzuki; Yasuko Matsunaga; Shunz 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 377 KB

## Abstract Large‐scale screening for human parvovirus B19 (619) DNA in serum samples was carried out by both dot blot hybridization and the polymerase chain reaction (PCR). Dot blot hybridization was undertaken with a digoxigenin‐labeled DNA probe. Serum samples from four patients were pooled and

Human papillomavirus infection of the ma
Human papillomavirus infection of the male diagnosed by Southern-blot hybridization and polymerase chain reaction: Comparison between urethra samples and penile biopsy samples
✍ Dr. Akio Kataoka; Urban Claesson; Bengt Göran Hansson; Margareta Eriksson; Elisa 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 885 KB

## Abstract A group of 108 healthy men aged 18 to 23 years underwent penoscopy after application of acetic acid. In 39 (37%) aceto‐white lesions were found and a biopsy was taken. From 105 patients a urethra sample was collected with a brush. The prevalence of HPV‐DNA by dot‐blot and Southern‐blot

Colposcopy, punch biopsy, in situ DNA hy
Colposcopy, punch biopsy, in situ DNA hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (HPV) infections in women with normal PAP smears
✍ Dr. Stina Syrjänen; Jukka Saastamoinen; Fuju Chang; Hongxiu Ji; Kari Syrjänen 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 835 KB

## Abstract To assess the prevalence of HPV infection in the genital tracts of women with normal PAP smears, a random series of 109 women was reexamined using colposcopy, a further PAP smear, and punch biopsies taken from the cervix (in 33 cases) vagina (212 cases), and anus (20 cases). The biopsy

Time trends in the prevalence of human p
Time trends in the prevalence of human papillomavirus infections in archival Papanicolaou smears: Analysis by cytology, DNA hybridization, and polymerase chain reaction
✍ Dr. Piroska Rakoczy; Gregory Sterrett; Jerzy Kulski; Darrel Whitaker; Laura Hutc 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 891 KB

## Abstract A retrospective study was undertaken to determine the prevalence of human papillomavirus (HPV) infections in routine Papanicolaou (Pap) smears collected by general practitioners from Western Australian women in each of the years 1972, 1982, and 1987. HPV infection was detected by cytolo

Detection of human T-lymphotrophic virus
Detection of human T-lymphotrophic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH)
✍ Koichi Ohshima; Junji Suzumiya; Shuji Izumo; Yasuo Mukai; Kohtaro Tashiro; Masah 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 727 KB

To examine the relationshiD between human T-lvmphotro-MATERIAL AND METHODS phic virus type I (HTLV-I) prov/ral DNA and its expreision in the lymph nodes, HTLV-I DNA and tax/rex mRNA were directly amplified by polymerase chain reaction in situ hybridization (PCRASH), and reverse transcription (RT)-PC