Fifty eight mitochondrial mutants (p + mit- mutants), all deficient in cytochrome oxidase activity and previously assigned to the genetic region oxi3 on the mitochondrial DNA, were mapped by the method of "petite deletion mapping". This procedure resulted in the identification of at least twenty one
Genetics of oxidative phosphorylation: Petite deletion mapping of the Oli 2 region of the mitochondrial genome of Saccharomyces cerevisiae
β Scribed by Connerton, I. F. ;Ray, M. K. ;Lancashire, W. E. ;Griffiths, D. E.
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 364 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
β¦ Synopsis
Petite deletion mapping has been carried out for the Oli 2 region of the mitochondrial genome of Saccharomyces cerevisiae to produce a fine structure genetic map. Previously unlocated mit- mutants together with the drug resistant loci Oli 2 and Oss 1 have been ordered between the cytochrome oxidase and apocytochrome b genes. As a result of this study a series of isogenic p- clones have been isolated spanning the Oli 2 region.
π SIMILAR VOLUMES
A polypeptide chain-terminating mutation (M5631) previously has been shown to be a +1T insertion in the yeast mitochondrial gene oxi1, coding for subunit II of the cytochrome c oxidase. A spontaneously arisen frameshift suppressor (mfs-1) that is mitochondrially inherited suppresses this mutation to