Genetically engineered V79 Chinese hamster cells for stable expression of human cytochrome P450IA2
✍ Scribed by Wölfel, Catherine; Heinrich-Hirsch, Barbara; Schulz-Schalge, Thomas; Seidel, Albrecht; Frank, Heinz; Ramp, Uwe; Wächter, Felix; Wiebel, Friedrich J.; Gonzalez, Frank; Greim, Helmut; Doehmer, Johannes
- Book ID
- 122414490
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 920 KB
- Volume
- 228
- Category
- Article
- ISSN
- 0926-6917
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In continuation of our work toward the establishment of a working cell bank for metabolic and toxicological studies, V79 Chinese hamster cells were genetically engineered for stable expression of rat cytochrome P450IA2. Full-length cDNA encoding rat P450IA2 was obtained by searching a cDNA library m
Chinese hamster V79 fibroblasts, frequently used as target cells in short-term tests for mutagenicity, do not possess measurable monooxygenase activity; in particular, enzymatic oxidation of testosterone (T) cannot be demonstrated. If these V79 cells, however, had been transfected with the cDNAencod