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GENETIC VARIATION FOR ANTIBIOTIC PERSISTENCE IN ESCHERICHIA COLI

โœ Scribed by Balint Stewart; Daniel E. Rozen

Book ID
109287393
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
639 KB
Volume
66
Category
Article
ISSN
0014-3820

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Several thousand mutagenized clones of Escherichia coli were screened for methyl group incorporation into protein in crude extracts, in order to isolate mutants lacking the full complement of methyl groups in ribosomal proteins. One mutant isolated by this method and designated prm-1 incorporated 6-

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The ribosomes of an Escherichia coli mutant, designated prm-2, can be methylated in vitro by an enzymatic fraction from wild-type. This enzyme is inactive on the ribosomes from another mutant, prm-1, is reported previously to be methyl group-deficient in protein L11. In vitro methylation of prm-2 ri