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Genetic transformation of Saccharomyces cerevisiae with single-stranded circular DNA vectors

✍ Scribed by Singh, Harinder; Bicker, James J.; Dumas, Lawrence B.

Book ID
123105940
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
1022 KB
Volume
20
Category
Article
ISSN
0378-1119

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The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and