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Genetic transformation and biotechnological application of the yeastArxula adeninivorans

✍ Scribed by T. Wartmann; G. Kunze


Publisher
Springer
Year
2000
Tongue
English
Weight
185 KB
Volume
54
Category
Article
ISSN
1432-0614

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The ILV1 gene of the yeast Arxula adeninivorans LS3 (AILV1) has been cloned from a genomic library, characterized and used as an auxotrophic selection marker for transformation of plasmids into this yeast. One copy of the gene is present in the Arxula genome, comprising 1653 bp and encoding 550 amin

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Abbreviations Aga Ξ±-agglutinin AOX1 alcohol oxidase I BPTI bovine pancreatic trypsin inhibitor ER endoplasmic reticulum GAP glyceraldehyde-3-phosphate dehydrogenase PDI protein disulfide isomerase scFv single-chain antibody variable region fragment