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Cloning and characterization of aTEFgene for elongation factor 1α from the yeastArxula adeninivorans

✍ Scribed by Harald Rösel; Gotthard Kunze

Publisher: Springer-Verlag
Year: 1995
Tongue: English
Weight: 818 KB
Volume: 28
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00326434

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✦ Synopsis

The translation elongation factor EF-1 alpha appears to play a major role in the control of cell proliferation and ageing in higher eukaryotes. Here we report the cloning of the TEF1 gene encoding the elongation factor 1 alpha of the dimorphic yeast Arxula adeninivorans Ls3. The gene is localized on chromosome 2 from Arxula adeninivorans, comprises 1380 bp and encodes a protein containing 459 amino acids. In contrast to other fungi, a second TEF gene encoding an identical, or nearly identical, polypeptide could not be identified. The transcriptional activity of the TEF1 gene did not change during mycelial growth, whereas a slight decrease could be detected during the yeast growth.

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