๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Genetic selection of lambda phage clones from a gene clonotheque

โœ Scribed by Melnikov, Anatolij A. ;Fodor, Istvan

Book ID
104727088
Publisher
Springer
Year
1986
Tongue
English
Weight
442 KB
Volume
203
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

โœฆ Synopsis

A genetic procedure for selection of specific lambda clones, by homologous recombination between lambda clones from a gene clonotheque and sequences cloned into a plasmid, was developed. Resulting clones are isolated in transduction experiments by plating infected Escherichia coli cells under conditions selecting for the antibiotic resistance marker carried by the plasmid. The feasibility of the method was demonstrated in a model test system as well as by isolation of alpha-interferon-specific sequences from the human gene clonotheque.

๐Ÿ“œ SIMILAR VOLUMES

Lambda phage promoter used to enhance ex
Lambda phage promoter used to enhance expression of a plasmid-cloned gene
โœ Hedgpeth, Joe ;Ballivet, Marc ;Eisen, Harvey ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 690 KB

A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and