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Lambda phage promoter used to enhance expression of a plasmid-cloned gene

✍ Scribed by Hedgpeth, Joe ;Ballivet, Marc ;Eisen, Harvey

Book ID: 104692978
Publisher: Springer
Year: 1978
Tongue: English
Weight: 690 KB
Volume: 163
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00267410

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✦ Synopsis

A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.

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