β Scribed by C. Goldthwaite, D. Dubnau and I. Smith
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Resistance to virginiamycin S (VS, a type B synergimycin) has been mapped in Bacillus subtilis. Transduction experiments with phage PBS1 indicate that the gene for virginiamycin S resistance (VSR) is cotransduced with the markers pur A16 (20%) and cys A14 (46-49%). Transformation experiments indicat
Among spontaneously occurring antibiotic-resistant mutants of Bacillus subtilis 168 we have identified a sub-class that is conditionally sporulative. Mutants in this sub-class are resistant to antibiotic during vegetative growth but are sensitive during sporulation. Mutants conditionally-resistant t
A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected.
Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas