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Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems

✍ Scribed by Nina Bandmann; Eric Collet; Jeanette Leijen; Mathias Uhlén; Andres Veide; Per-Åke Nygren

Book ID
119523791
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
368 KB
Volume
79
Category
Article
ISSN
0168-1656

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✍ M. J. Sebastião; J. M. S. Cabral; M. R. Aires-Barros 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 289 KB

An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecu