Sucrose synthetase activity in endosperm extracts of seven shrunken(sh) mutants of spontaneous origin and three similar mutants due to the association of the controlling element Ds with the Sh locus is examined. A residual level of 3 to 5% as compared to the normal (Sh) endosperm is seen in all the
Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize
โ Scribed by H. K. Dooner; O. E. Nelson
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 552 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0006-2928
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โฆ Synopsis
The enzyme UDPglucose:flavonol 3-O-glucosyltransferase is shown to be under the coordinate control of three genes involved in anthocyanin biosynthesis in the aleurone of maize: C, R, and BZ. Of the three, BZ appears to be the structural gene. Data presented here (dosage comparisons, induction in the mutant c-p, and effect of paramutation at R) indicate that the enzyme is inducible by substances resulting from the action of the C and R genes and that active forms of C and R are required for this induction. Mechanisms of regulation of the BZ gene by C and R are discussed.
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An association is reported between an albuminlike protein (b-70) and the semidominant locusfluory-2 (172) which reduces the level of zein polypeptides in the maize endosperm. The protein b-70 is present at low level in wildtype endosperms and derepressed infl2 endosperms. A correlation between the d
An anthocyanin 5-O-glucosyltransferase from flowers of Petunia hybrida was purified about 30-fold. Using uridine 5'-diphosphoglucose as glucose donor (Km 0.22 mM), the enzyme glucosylated the 3-(p-coumaroyl)-rutinoside derivatives of delphinidin and petunidin (Km 3 gM), isolated from pollen of Petun