Genetic control of glucose uptake by Escherichia coli
β Scribed by H.L. Kornberg; Janet Smith
- Book ID
- 115902563
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 249 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0014-5793
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The fluorescent glucose analog, 2-(N-(7-nitrobenz-2-oxa-1,3diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was used to measure rates of glucose uptake by single Escherichia coli cells. When cell populations were exposed to the glucose analog, 2-NBDG was actively transported and accumulated in single cel
## Abstract In contrast to active transport, the uptake of carbohydrates via the phosphoenolpyruvateβdependent phosphotransferase system (PTS) leads to the appearance in the cell of the sugar initially as a 1β or 6β phosphate ester. The components of the PTS that transfer phosphate to the sugar are