Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10
โ Scribed by Jack H. Staddon; Edward M. Bryan; Dawn A. Manias; Yuqing Chen; Gary M. Dunny
- Book ID
- 113855277
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 253 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0147-619X
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๐ SIMILAR VOLUMES
The entire region required for mobilization of the non-conjugative plasmid RSF1010 has been cloned into a mobilization-deficient pBR322 derivative. The segment of DNA cloned was approximately 1.8 kb and included the origin of conjugal DNA transfer (oriT). The DNA sequence of the mobilization region
The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a