Genetic analysis ofopaque2modifier gene activity in maize endosperm
โ Scribed by M. A. Lopes; B. A. Larkins
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 912 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0040-5752
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Sucrose synthetase activity in endosperm extracts of seven shrunken(sh) mutants of spontaneous origin and three similar mutants due to the association of the controlling element Ds with the Sh locus is examined. A residual level of 3 to 5% as compared to the normal (Sh) endosperm is seen in all the
Sodium dodecylsulfate-polyacrylamide gel electrophoresis reveals that zein prepared from normal maize inbred (Zea mays L.) contains six separable components. Z1 and Z2 are the predominant species, with molecular weights of 21,800 and 19,000 daltons. Amino acid analysis of these two components shows