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Genetic analysis of thiopurine methyltransferase polymorphism in the Jordanian population

✍ Scribed by Nancy Hakooz; Tawfiq Arafat; Debbie Payne; William Ollier; Sudeep Pushpakom; Julie Andrews; William Newman


Publisher
Springer
Year
2010
Tongue
English
Weight
113 KB
Volume
66
Category
Article
ISSN
0031-6970

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To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of the gene amplified by polymerase chain reaction (PCR). The sensitivity of the method was first evaluated by analyzing DNA samples fr