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Genetic Analysis, by means of Transformation, of Histidine Linkage Groups in Bacillus Subtilis

✍ Scribed by Erela Ephrati-Elizur, P. R. Srinivasan and Stephen Zamenhof

Book ID
123656222
Publisher
National Academy of Sciences
Year
1961
Tongue
English
Weight
346 KB
Volume
47
Category
Article
ISSN
0027-8424

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Genetic mapping by means of protoplast f
Genetic mapping by means of protoplast fusion in Bacillus subtilis
✍ Akamatsu, T. ;Sekiguchi, J. πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 903 KB

A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected.