Generation of soluble and active subtilisin and α-chymotrypsin in organic solvents via hydrophobic ion pairing
✍ Scribed by MEYER, JEFFREY D. ;KENDRICK, BRENT S. ;MATSUURA, JAMES E. ;RUTH, JAMES A. ;BRYAN, PHILIP N. ;MANNING, MARK C.
- Book ID
- 115099949
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 378 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0367-8377
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## Abstract A new technique for enhancing the solubility of peptides and proteins in organic solvents is described. Complexation of polypeptides with stoichiometric amounts of an anionic detergent, such as sodium dodecyl sulfate (SDS), produces diminished aqueous solubility, but enhanced solubility
## Abstract Enzymes are attractive catalysts for the production of optically active compounds in organic solvents. However, their often low catalytic activity in such applications hampers their practical use. To overcome this, we investigated the effectiveness of the covalent modification of α‐chym