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Generation of region-specific antibodies to modified proteins

✍ Scribed by Linda K. Curtiss; Urs P. Steinbrecher; Joseph L. Witztum

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
610 KB
Volume
1
Category
Article
ISSN
0887-8013

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✦ Synopsis

Nonenzymatic modifications of the Eamine of lysine residues in proteins have been described in diabetes, uremia, highdose aspirin therapy, and alcoholism. In this review, we describe the generation of lysine-modification-specific antibodies that can be used to identify and to quantitate nonenzymatic glucosylation, carbamyla-tion, acetylation, ethylation, or methylation of lysine residues. Polyvalent or monoclonal antibodies that are absolutely specific for the lysine-specific modification are obtained by immunization with modified autologous or homologous low-density plasma lipoproteins.

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Abbreviations 9cRA 9-cis retinoic acid atRA all-trans retinoic acid ER estrogen receptor GR glucorticoid receptor NHR nuclear hormone receptor PR progesterone receptor RAR retinoic acid receptor RXR retinoid X receptor