Electroblotting of proteins on agarose gel containing specific antibodies
β Scribed by Dr. Ragheb F. Atmeh; Nizar M. Abuharfeil
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 481 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
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A fast and convenient method for silver staining of proteins on electroblotting membranes was developed based on Gallyas' histochemical intensifier and applied to human endothelial cell proteins separated by one-and two-dimensional electrophoresis and electroblotted to polyvinyl difluoride membranes
A gel matrix composed of 0.5 % agarose and 2.5 % polyacrylamide allows the parallel electrophoretic separation of nonreduced IgM and IgG monoclonal antibodies. The mobilities of IgM and IgG differ at pH 9.5, which enables apreliminary Ig class designation. It allows rapid detection of incomplete clo