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Electrophoresis of nonreduced IgM and IgG monoclonal antibodies on mixed agarose-polyacrylamide gels

✍ Scribed by Dr. Alena Slavíčková

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 149 KB
Volume: 11
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150111020

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✦ Synopsis

A gel matrix composed of 0.5 % agarose and 2.5 % polyacrylamide allows the parallel electrophoretic separation of nonreduced IgM and IgG monoclonal antibodies. The mobilities of IgM and IgG differ at pH 9.5, which enables apreliminary Ig class designation. It allows rapid detection of incomplete cloning ifboth IgM and IgG are present. A semiquantitative evaluation of nonreduced IgM in ascitic fluid is possible as well.

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