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Generation of genetically engineered CHO cell lines to support the production of a difficult to express therapeutic protein

✍ Scribed by Laux, Holger; Romand, Sandrine; Ritter, Anett; Oertli, Mevion; Fornaro, Mara; Jostock, Thomas; Wilms, Burkhard


Book ID
121591464
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
273 KB
Volume
7
Category
Article
ISSN
1753-6561

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A CHO cell line engineered to express XB
✍ Cain, Katharine; Peters, Shirley; Hailu, Hanna; Sweeney, Bernie; Stephens, Paul; πŸ“‚ Article πŸ“… 2013 πŸ› American Institute of Chemical Engineers 🌐 English βš– 585 KB

Transient gene expression (TGE) systems currently provide rapid and scalable (up to 100 L) methods for generating multigram quantities of recombinant heterologous proteins. Product titers of up to 1 g/L have been demonstrated in HEK293 cells but reported yields from Chinese hamster ovary (CHO) cell