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Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors

โœ Scribed by A. Gruber; I. Chen; K.L. Kuhen; J.C. Wheat; P. Law; F. Wong-Staal

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
108 KB
Volume
70
Category
Article
ISSN
0146-6615

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โœฆ Synopsis

Abstract

Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34^+^ cells from HIVโ€infected subjects can be transduced with a replicationโ€incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34^+^ cells from HIVโ€infected individuals were able to differentiate into dendritic cells with strong Tโ€cell stimulatory capacity. Thus, cryopreserved CD34^+^ cells from HIVโ€infected subjects may prove useful for immunotherapy for HIV/AIDS. J. Med. Virol. 70: 183โ€“186, 2003. ยฉ 2003 Wileyโ€Liss, Inc.

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