Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine

✍ Scribed by David Shrayer; Nicolas Kouttab; Abby Maizel; Harold Wanebo; Vincent J. Hearing; Douglas M. Gersten

• Book ID: 102867853
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 967 KB
• Volume: 53
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910530428

✦ Synopsis

The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor-challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from 6 I6 F I 0 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross-reactivity with the 6700 melanoma.antigen. The antibodies were shown to be anti-proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement-mediated and antibodydependent cellular cytotoxicity. Flow cytometric analyses indicated that -60% of the target cells specifically bound antibody from the immune sera. These results confirm that 6700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.