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Generation of chemiluminescence during enzymatic isolation of protoplasts from leaves of Oryza sativa

✍ Scribed by Adrian D. Butt; Charles S. Bestwick

Book ID
117392383
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
603 KB
Volume
150
Category
Article
ISSN
0176-1617

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Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in