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Generation of a Novel High-Affinity Monoclonal Antibody with Conformational Recognition Epitope on Human IgM

✍ Scribed by Sina Sarikhani; Manouchehr Mirshahi; Mohammad Reza Gharaati; Tooran Mirshahi


Book ID
107565551
Publisher
Springer-Verlag
Year
2010
Tongue
English
Weight
238 KB
Volume
162
Category
Article
ISSN
0273-2289

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## Abstract Human mknoclonal antibodies were produced against __Mycoplasma hominis__ by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). The immune B lymphocytes were subsequently immortalized by Epstein‐Barr virus trans