A high-affinity human monoclonal igm antibody reacting with multiple strains of mycoplasma hominis
✍ Scribed by Susanna A. Möller; Carl A. K. Borrebaeck; Svend Birkelund
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 578 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Human mknoclonal antibodies were produced against Mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). The immune B lymphocytes were subsequently immortalized by Epstein‐Barr virus transformation followed by somatic cell fusion. Using this procedure, several specific anti‐M. hominis monoclonal antibodies of μ isotype were obtained exhibiting apparent affinity constants as high as 3 × 10^9^ M^−1^. The productivity of a number of different hybridomas was determined to be in the range 7‐22 μg × (24 hr × 10^6^ cells)^−1^. The specificity of one antibody, 129.1, was further characterized by enzyme‐linked immunosorbent assay against several different species of Mycoplasma and by Western blot analysis. The antibody specifically bound to a protein of M, 100,000 present in 10 of 12 different strains of M. hominis.