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Generation and Characterization of a Stable Soluble Guanylate Cyclase-Overexpressing CHO Cell Line

✍ Scribed by Eva Maria Becker; Frank Wunder; Raimund Kast; Chantal Robyr; Markus Hoenicka; Rupert Gerzer; Henning Schröder; Johannes-Peter Stasch


Book ID
115641993
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
163 KB
Volume
3
Category
Article
ISSN
1089-8603

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## Abstract The proteolytic activities expressed by a Chinese hamster ovary (CHO) cell line in the cultivation supernatant during the production of recombinant factor VIII were mapped with a broad spectrum protease assay and a series of different types of protease inhibitors. The destabilizing effe