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General method of preparation of uniformly13C,15N-labeled DNA fragments for NMR analysis of DNA structures

✍ Scribed by Brigitte René; Grégoire Masliah; Loussiné Zargarian; Olivier Mauffret; Serge Fermandjian

Book ID
106401401
Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
416 KB
Volume
36
Category
Article
ISSN
0925-2738

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Determination of Homo- and Heteronuclear
Determination of Homo- and Heteronuclear Coupling Constants in Uniformly 13C,15N-Labeled DNA Oligonucleotides
✍ D. P. Zimmer; J. P. Marino; C. Griesinger 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 805 KB

A set of experiments to determine homo-and heteronuclear vicinal coupling constants in uniformly 13C-labeled DNA oligonucleotides is presented. 2D and 3D HCCH-E.COSY experiments were used to measure 3Jm coupling constants in the deoxyribose ring, a refocused HMBC experiment was used to measure 3Jm c