General evaluation and application to trace analysis of a chiral column for ligand-exchange chromatography

✍ Scribed by Hideaki Kiniwa; Yuka Baba; Tomoko Ishida; Hiromasa Katoh

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 533 KB
• Volume: 461
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94306-1

✦ Synopsis

The direct optical resolution of more than twenty amino acids, including the separation of four isomers of allo-DL-and Dr+-isoleucine, can be achieved on a novel ligand-exchange column, MCI GEL CRSlOW. The stationary phase, N,N-dioctyl+ alanine-coated octadecylsilica, shows a stronger affinity for hydrophobic amino acids than for hydrophilic amino acids. General evaluation of the column clarified the effects of the column temperature and Cu" concentration in the eluent on the chiral separation. The determination of trace enantiomers in some commercially available D-and L-amino acids was performed, revealing the presence of 0.0345% of the trace enantiomer. The efficient resolution of strongly retained hydrophobic amino acids and hydroxycarboxylic acids was also achieved on a shortened column.