General discussion on transplantation of bone marrow

• Publisher: Wiley (John Wiley & Sons)
• Year: 1957
• Tongue: English
• Weight: 506 KB
• Volume: 50
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030500412

✦ Synopsis

The minimum 30-day LD,,, f o r this strain of mice is about 575r.

COLE: You see no early deaths in the controls at this dose. By early, I mean about thc first week.

hIAKINODAN : Death takes place 5-15 days after irradiation.

COLE:

My point was that, at least in our hands, in this general range of dose, there is a bimodal death response the first part of which corresponds to the "intestinal mode of death."

MAKINODAN: I believe it is just the difference in strains of mice. COLE: Another point I should like to raise is Dr. Loutit's reference to the identification of donated cells in thymus and lymph tissue. From our results using the alkaline-phosphatase technique as a marker for the fate of the injected rat bone marrow cells and also some other attempts to assay the proclivity for cell division of injected thymus cells, it appears that the regeneration or response in the thymus is secondary to the regeneration of the bone marrow. Thus I am not sure that we can speak about the donated thymus cells o r lymphocytes, particularly since the injection of thymus cells or lymphocytes alone provides no protection against LD,,, doses in mice, and also since there is no evidence that the injection of thymus or lymphoid cells in the peritoneal cavity in irradiated mice is followed by multipli-