Gene therapy by intercellular trafficking of p53-VP22 induces regression of orthotopic hepatocellular carcinoma in rats
✍ Scribed by Lars Zender; Sebastian Hütker; Bernd Frericks; Matthias Eckhard; Thomas Gösling; Michael Galanski; Christian Krettek; Florian Kühnel; Michael Manns; Stefan Kubicka
- Book ID
- 118565212
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 214 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0168-8278
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## Abstract Immunohistochemical, immunoblotting, and DNA‐sequencing analyses were performed on hepatocellular carcinomas induced in rats chronically fed BR931, a peroxisome proliferator, to determine whether the tumors carried mutations or other alterations of the __p53__ gene. None were detected.
## Abstract Structural alterations of the __p53__ gene were investigated in chemically induced rat hepatocellular carcinomas (HCCs), hyperplastic hepatic nodules (HPNs), and cell lines derived from rat neoplastic and normal liver cells. The mutations were detected by GC‐clamped denaturing gradient