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Gene synthesis by circular assembly amplification

✍ Scribed by Bang, Duhee; Church, George M


Book ID
109937708
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
226 KB
Volume
5
Category
Article
ISSN
1552-4450

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In this work, we have studied the effect of amplifying different alleles involved in the threonine biosynthesis on the amino acid production by Saccharomyces cerevisiae. The genes used were wild-type HOM3, HOM2, HOMS, THR7, and THR4, and two mutant alleles of HOM3 (namely HOM3-RZand HOM3-R6), that c