Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley
β Scribed by Somssich, Imre E. ;Schmelzer, Elmon ;Kawalleck, Petra ;Hahlbrock, Klaus
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 690 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
We have analysed three nearly full-length cDNAs complementary to mRNAs encoding two PR1 (pathogenesis-related, class 1) proteins in parsley (Petroselinum crispum). Furthermore, one selected genomic clone containing the PcPR1-1 gene was investigated in detail. The structural organization and possible regulatory elements in the 5' flanking region of this gene are presented. In situ RNA hybridization in fungus-infected parsley leaf tissue demonstrated rapid and massive PR1 mRNA accumulation around infection sites.
π SIMILAR VOLUMES
A cDNA clone, RhIXb (1384bp), encoding the entire protein sequence of a human blood group Rh polypeptide has been used to map the Rh locus, by in situ hybridization, to the region p34.3-p36.1 of chromosome 1. Two other unrelated cDNA clones, pUCA2 (750bp) and pUCIII (1600 bp), isolated during the cl