We have developed a simple and efficient method to construct partial libraries of swine Chromosome (Chr) 11, starting with only 300 flow-sorted copies. DNA is amplified by PARM-PCR with primer containing at the 5'-end the sequence AGCU-. After amplification, digestion of PCR products with uracil DNA
Gene mapping by enzymatic amplification from flow-sorted chromosomes
โ Scribed by Cotter, Finbarr; Nasipuri, Soma; Lam, Grace; Young, Bryan D.
- Book ID
- 123116251
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 1022 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0888-7543
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