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Gene expression profiling of liver cells after copper overload in vivo and in vitro reveals new copper-regulated genes

✍ Scribed by Patricia Muller; Harm van Bakel; Bart van de Sluis; Frank Holstege; Cisca Wijmenga; Leo W. J. Klomp


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
422 KB
Volume
12
Category
Article
ISSN
1432-1327

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We inserted a zinc-responsive metallothionein-rasT24 fusion gene in both orientations into a retroviral vector (SVX) and infected Fisher rat liver epithelial cells. Only the construction in which the viral long terminal repeat and the metallothionein promoters were in opposite orientations resulted