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Gene expression of human T lymphocytes cell cycle: Experimental and bioinformatic analysis

✍ Scribed by Luca Giacomelli; Claudio Nicolini

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
253 KB
Volume
99
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

Human lymphocytes gene expression is monitored before and after PHA stimulation over 72 h, using DNA microarray technology. Results are then compared with our previous bioinformatics predictions, which identified six leader genes of highest importance in human T lymphocytes cell cycle. Experimental data are strikingly compatible with bioinformatic predictions of the specific role and interaction of PCNA, CDC2, and CCNA2 at all phases of the cell cycle and of CHEK1 in regulating DNA repair and preservation. It does not escape our notice that the conception and use of ad hoc arrays, based on a bioinformatics prediction which identifies the most important genes involved in a particular biological process, can really be an added value in cell biology and cancer research alternative to massive frequently misleading molecular genomics. J. Cell. Biochem. 99: 1326–1333, 2006. Β© 2006 Wiley‐Liss, Inc.

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