✦ LIBER ✦

Gene expression in the cell cycle of human T-lymphocytes: II. Experimental determination by DNASER technology

✍ Scribed by Claudio Nicolini; Rosanna Spera; Enrico Stura; Stefano Fiordoro; Luca Giacomelli

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 182 KB
Volume: 97
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.20694

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human lymphocytes gene expression before and after PHA stimulation is monitored by DNASER technology, a novel bioinstrumentation entirely constructed in our laboratories as previously reported. The validity of the DNASER measurements is confirmed by standard fluorescence microscopy equipped with CCD. The human lymphocytes gene expression here experimentally probed using commercially available DNA microarrays such as Human Starter, appears compatible both with independent bioinformatic prediction and with existing experimental data, pointing to MYC as the key gene in the G~0~–G~1~ transition induced by PHA in resting lymphocytes. It does not escape our notice that in cell biology and cancer research DNASER technology based on microarray constructed with few leader genes identified from bioinformatics represents a meaningful cost‐effective route alternative to massive frequently misleading molecular genomics. J. Cell. Biochem. 97: 1151–1159, 2006. © 2005 Wiley‐Liss, Inc.

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