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Gene cloning, expression, and crystallization of a thermostable exo-inulinase fromGeobacillus stearothermophilusKP1289

✍ Scribed by Y. Tsujimoto; A. Watanabe; K. Nakano; K. Watanabe; H. Matsui; K. Tsuji; T. Tsukihara; Y. Suzuki

Publisher
Springer
Year
2003
Tongue
English
Weight
308 KB
Volume
62
Category
Article
ISSN
1432-0614

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Cloning and expression of a thermostable
Cloning and expression of a thermostable exo-Ξ±-1,4-glucosidase gene fromBacillus stearothermophilusATCC12016 inEscherichia coli
✍ Yukio Takii; Katsuya Daimon; Yuzuru Suzuki πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 856 KB

The gene coding for a thermostable exo-alpha-1,4-glucosidase (alpha-glucoside glucohydrolase: EC 3.2.1.20) of Bacillus stearothermophilus ATCC 12016 was cloned within a 2.8-kb AvaI fragment of DNA using the plasmid pUC19 as a vector and Escherichia coli JM109 as a host. E. coli with the hybrid plasm