Gel permeation chromatography—II. The effect of solvent flow rate on the calculated molecular weight averages

✍ Scribed by A.R. Cooper; J.F. Johnson; A.R. Bruzzone

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 408 KB
• Volume: 9
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(73)90109-2

✦ Synopsis

The experimental data reported previously have been used to calculate the differential molecular weight distribution of the samples as a function of the solvent flow rate. These calculations were performed without applying a dispersion correction and also with a dispersion correction method called "Reshaping". The effect of flow rate on molecular weight averages has been determined. The resolution parameter, relating observed to ideal behavior, has been found to be molecular weight dependent at 1'0 ml/min flow rate but independent of molecular weight at 0-2 ml/min flow rate.

With this polystyrene gel column, the efficiency was greater when tetrahydrofuran, rather than toluene, was used as solvent, at similar flow rates.