Gel permeation chromatography: The effect of flow rate on efficiency. II

✍ Scribed by Jau-Yi Chuang; Julian F. Johnson; Anthony R. Cooper

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 483 KB
• Volume: 28
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1983.070280204

✦ Synopsis

Abstract

The effect of flow rate on efficiency in gel permeation chromatoraphy has been examined over wide ranges of flow rate, i.e., linear flow velocities using columns of different diameters using both organic and inorganic packings. The results indicate a considerable increase in efficiency at flow velocities below 0.02 cm/s. No lower limit to this increase could be determined due to experimental difficulties. Between flow velocities of 0.02–0.2 cm/s little efficiency is lost.