Aqueous gel permeation chromatography: The effect of solvent ionic strength

✍ Scribed by Anthony R. Cooper; David P. Matzinger

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 391 KB
• Volume: 23
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1979.070230211

✦ Synopsis

Abstract

Porous glass packing materials have been used for gel permeation chromatography using an aqueous phosphate buffer. Elution volumes were determined for polystyrene sulfonates, dextrans, and small neutral and charged molecules at three different ionic strengths, viz., 0.01__M__, 0.1__M__, and 1.0__M__ phosphate, pH 7.0. The pore diameters of the glasses studied were 75, 240, 700, and 2000 Å. Elution volumes of nonionic species were unaffected by changing the solvent ionic strength. Elution volumes of charged species were markedly affected by the ionic strength of the solvent. This was attributed to a combination of decreased polymer dimensions and decreased ionic exclusion with increasing buffer concentration. The use of low ionic strength solvents may be exploited to tailor the separating range for polyelectrolytes with porous glass packings. This is particularly useful in the low molecular weight range where the lowest pore size available is 75 Å.