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GC determination of pilocarpine and isopilocarpine

✍ Scribed by Hassan Y. Aboul-Enein

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
107 KB
Volume
65
Category
Article
ISSN
0022-3549

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The 2OOMHz 'H NMR spectra of pilocarpine, pilocarpine.HC1 and isopilocarpine.HC1 have been studied. Evidence is presented for the preferred conformations of the compounds in aqueous solution, with the methylimidazolyl moiety antiperiplanar with respect to the a-carbon of the butanolide ring.

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A normal-phase high-performance liquid chromatographic separation for pilocarpine and isopilocarpine was developed which is suitable for the routine analysis of ophthalmic preparations. The method utilizes a column packed with 5 -~m silica with a mobile phase of hexane-2% ammonium hydroxide in 2-pro