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Gas-phase hydrogen–deuterium exchange in phosphorothioate d(GTCAG) and d(TCGAT)

✍ Scribed by Richard H. Griffey; Michael J. Greig; Jessica M. Robinson; David A. Laude

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
93 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

The gas-phase hydrogen-deuterium exchange of phosphorothioate d(GTCAG) and d(TCGAT) has been examined using electrospray Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. The exchange has been monitored for the [M-2H] 2À , [M-3H] 3À , and [M-4H] 4À charge states. The mole fractions of each partially exchanged species have been calculated as a function of time. The rates of initial exchange could not be fit to simple exponential functions. The results suggest that each ion can adopt more than one gas-phase conformation, and that these conformers exchange at different rates. Observation of slowly interconverting populations is consistent with a model where initial ion conformations contain internal hydrogen bonds between the bases and charged residues that are disrupted over time.

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