Gas-Phase Concentration, Purification, and Identification of Whole Proteins from Complex Mixtures
โ Scribed by Reid, Gavin E.; Shang, Hao; Hogan, Jason M.; Lee, Gil U.; McLuckey, Scott A.
- Book ID
- 125471981
- Publisher
- American Chemical Society
- Year
- 2002
- Tongue
- English
- Weight
- 122 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0002-7863
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๐ SIMILAR VOLUMES
A substantial degree of complexity is conferred upon the proteome by the posttranslational modification of proteins. One of the hallmarks of the proteome is its ability to dynamically regulate protein expression in response to internal and external stress. The stress can be physiological, pathologic
Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100