✦ LIBER ✦

Gas-phase cationization and protonation of neutrals generated by matrix-assisted laser desorption

✍ Scribed by Bing H. Wang; Klaus Dreisewerd; Ute Bahr; Michael Karas; Franz Hillenkamp

Book ID: 103995693
Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 632 KB
Volume: 4
Category: Article
ISSN: 1044-0305
DOI: 10.1016/1044-0305(93)85004-h

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✦ Synopsis

The ionization mechanisms involved in matrix-assisted ultraviolet laser desorption/ionization (MALDI) were studied with a time-of-flight mass spectrometer. When protonated or cationized quasimolecular ions generated by MALDI are not extracted promptly, their abundance is a function of the delay time between laser irradiation and ion extraction, maximizing at an optimum delay time (DTM) of a few hundred nanoseconds. The ion abundance at DTM exceeds that of prompt extraction by a factor of 2 or more. Increasing the cation density near the sample surface reduces the DTM, whereas increasing the desorption laser irradiance has the opposite effect. The enhancement suggests extensive gas-phase ion-molecule reactions after irradiation by the desorption laser has ceased.

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