Gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters of amino acids
β Scribed by S.L. MacKenzie; D. Tenaschuk
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 404 KB
- Volume
- 97
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
An excellent separation of the N-TFA N-butyl esters of seventeen of the protein amino acids was achieved with acid washed Chromosorb W as the support material mesh, dried at 140~ for 12 h). In these experiments, the liquid phase, stabilized EGA, was used at loadings of from 0.325 w/w o/0 to 0.65 w/w
Protein hydrolysates are now routinely analysed by ion exchange chromatography (1) but a complete determination takes at least 3 hours. In addition, the automated amino acid analyzers now used are expensive and have limited application to other problems. Gas chromatography (g.1.c.) offers potential