Gas-liquid chromatographic analysis of 2,4-dinitrophenylhydrazones of keto acid methyl esters
β Scribed by Heikki Kallio; Reino R. Linko
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 304 KB
- Volume
- 76
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
Fublishing Compnny, hrnstcrclntn
π SIMILAR VOLUMES
A practical method for the quantitative determination of protein amino acids by gas-liquid chromatography (GLC) is described. All of the common protein amino acids except arginine can be readily converted into their N-isobutyloxycarbonyl (N-isoBOC) methyl ester derivatives by a simple procedure invo
Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate