Gas chromatographic determination of the laxative 1,8-dihydroxyanthraquinone in urine and faeces

✍ Scribed by A.J. Baars; R.J. Vermeulen; D.D. Breimer

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 258 KB
• Volume: 120
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)99018-1

✦ Synopsis

1,8-Dihydroxyanthraquinone (danthron) is the basic structure of the aglycones of naturally occurring laxative glycosides, e.g. in the Cassia, Aloe, Rheum and Rhamnus s&es.

Danthron itself is in use as a synthetic laxative (Istizin@, Dorbane@)L-9.

In order to investigate the metabolic fate of danthron in the rat (to be published in full detail elsewhere), it was necessary to develop a method for analysing small amounts of danthron in faeces and urine.

The fluorescence method of Lane4 did not meet OUT expectations, possibly due to other substances in faeces and urine interacting with the Suorescence of the danthron moiety. We report here a procedure consisting of an extraction followed by adsorption chromatography (to purify the faeces extract) and gas chromatographic determination of danthron (without derivatisation): it allows the detection of nanogram quantities of danthron, undisturbed by other products excreted in faeces and urine.