Gas chromatographic determination of mebutamate, carisoprodol, and tybamate in plasma and urine

✍ Scribed by J. F. Douglas; N. B. Smith; J. A. Stockage

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 201 KB
• Volume: 58
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600580139

✦ Synopsis

of Mn2+. When sodium L-thyroxine was incorporated into the ergot culture medium, an appreciable decrease in alkaloid production resulted but an increase in growth was obtained. Thyroxine is known to inhibit cholesterol synthesis (22), a process which may be related to the effect of L-thyroxine on oxygen consumption. Evans (23), in discussing the mechanism of microbial hydroxylations, points to the involvement of oxygenases in these phenomena. One of the hydroxylase inhibitors is p-chlorophenylalanine. When it was incorporated into the medium, a marked decrease (52%) in alkaloid production resulted.

The results show, on one hand, that the incorporation of some oxygenase inducers into the ergot culture produces considerable increase in alkaloid production, while, on the other hand, reduction in alkaloid production is obtained from substances which have inhibitory effects on oxygenases. The findings, therefore, lend strong support to the concept that oxygenases are involved in the biogenesis of the ergot alkaloid.